Flexible metabolic pathway construction using modular and divisible selection gene regulators

Finding pathway regulators: gene set approach using peak identification algorithms

Recently, a number of different approaches have been used to examine variation in gene expression and to identify genes whose level of transcript differed greatly among unrelated individuals. Previous studies have commonly focused on identifying determinants that regulate gene expressions by targeting individual genes. However, it is difficult to detect true differences in the level of gene exp...

Selection, gene interaction, and flexible gene networks.

Recent results from a variety of different kinds of experiments, mainly using behavior as an assay, and ranging from laboratory selection experiments to gene interaction studies, show that a much wider range of genes can affect phenotype than those identified as "core genes" in classical mutant screens. Moreover, very pleiotropic genes can produce specific phenotypes when mild variants are comb...

Modular construction of mammalian gene circuits using TALE transcriptional repressors

An important goal of synthetic biology is the rational design and predictable implementation of synthetic gene circuits using standardized and interchangeable parts. However, engineering of complex circuits in mammalian cells is currently limited by the availability of well-characterized and orthogonal transcriptional repressors. Here, we introduce a library of 26 reversible transcription activ...

Construction of Group Divisible Designs

New Digital Fingerprint Code Construction Scheme Using Group-Divisible Design

Combinatorial designs have been used to construct digital fingerprint codes. Here, a new constructive algorithm for an anticollusion fingerprint code based on group-divisible designs is presented. These codes are easy to construct and available for a large number of individuals, which is important from a business point of view. Group-divisible designs have not been used previously as a tool for...